منابع مشابه
three-dimensional (3d) cell culture conditions, present and future improvements
conclusions our underlying hypothesis is that recapitulating the three-dimensional early embryonic structure, in order to reproduce better in vitro the three-dimensional morphogenetic-like re-arrangements, would improve cells differentiation, when in vivo transplanted; moreover, it could be used as a simplified cancer disease model and reliable drug evaluation method as well. results the proces...
متن کاملCulture and Assay Systems Used for 3D Cell Culture
Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...
متن کامل3D Cell Culture in Alginate Hydrogels
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choi...
متن کاملMicrofluidic Micropillar Arrays for 3D Cell Culture
Cell-based assays are one of the most important steps to select huge amount of drug candidates in drug discovery. To get more credible assay results, cell culture in the form of microscale environment and three-dimension has been exploited by microfluidic hydrodynamic focusing. However, the method still needs an enhanced reliability of scaffold formation and fast cell immobilization in a microc...
متن کاملAdvances in 3D neuronal cell culture
• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version ...
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ژورنال
عنوان ژورنال: European Journal of Histochemistry
سال: 2011
ISSN: 2038-8306,1121-760X
DOI: 10.4081/ejh.2011.br4